Fig. 2
From: Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness
Phenotypic analysis of RHΔtrx4. a The plaque assays of RH, RHΔtrx4 and RHΔtrx4C strains. b The relative plaque sizes of RH, RHΔtrx4 and RHΔtrx4C strains measured from (a). The experiments were performed three independent times. The difference was analyzed by Student’s t-test. ***P < 0.001; n.s., not significant. c Differences in the host cell invasion in RH, RHΔtrx4 and RHΔtrx4C strains. Five microscopic fields were randomly selected in each sample. The experiments were performed three independent times. The difference was analyzed by Student’s t-test. **P < 0.01; n.s., not significant. d Differences in the intracellular replication in RH, RHΔtrx4 and RHΔtrx4C strains grown in HFFs for 24 h. The experiments were performed three independent times. The difference was analyzed by Student’s t-test. n.s., not significant. e Differences in the egress between RH, RHΔtrx4 and RHΔtrx4C strains. The experiments were performed three independent times. The difference was analyzed by Student’s t-test. n.s., not significant