Fig. 1
From: Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness
The subcellular localization of Trx4 in Toxoplasma gondii. a Phylogenetic analysis of Trx4 proteins in T. gondii (TGME49_224060), Hammondia hammondi (HHA_224060), Neospora caninum (NCLIV_048460), Besnoitia besnoiti (BESB_079560), Cystoisospora suis (CSUI_002634), Eimeria tenella (EPH_0016720), Cyclospora cayetanensis (cyc_05007), Sarcocystis neurona (SN3_01700400), Cryptosporidium parvum (cgd1_800) and Plasmodium falciparum (PF3D7_1472600). The phylogenetic tree was constructed by the neighbor-joining method. b IFA revealed that Trx4 was localized in the PV. c and d Trx4 co-localized with GRA1 (c) or GRA5 (d). Green, rabbit anti-HA, mouse anti-GRA1 or rabbit anti-GRA5; red, mouse anti-IMC1, rabbit anti-HA or mouse anti-HA. Scale bar: 3 μm. e Western blotting confirmed that Trx4-6HA strain was successfully generated, and a single protein band was visualized. HA, rabbit anti-HA; ALD, rabbit anti-aldolase. f Trx4 was predicted to have two Trx domains, located at amino acid positions 71–195 and 475–593, respectively, as indicated by (https://prosite.expasy.org/)