An inside out journey: biogenesis, ultrastructure and proteomic characterisation of the ectoparasitic flatworm Sparicotyle chrysophrii extracellular vesicles

Table 3 List of Sparicotyle chrysophrii peptidases present in EV samples, showing the peptidase clan and family according to MEROPS database, including their best hit

Sparicotyle chrysophrii protein sequence	Protein type	Peptidase		Species	E-value
Sparicotyle chrysophrii protein sequence	Protein type	Clan	Family	Species	E-value
augustus_masked-contig_1681_pilon_pilon-processed-gene−0.0-mRNA-1	Leukotriene A-4 hydrolase^*	MA	M1	Schistosoma curassoni	1.90e−175
maker-contig_20098_pilon_pilon-augustus-gene−0.16-mRNA-1	Leucyl aminopeptidase	MF	M17	Fasciola spp.	5.20e−157
maker-contig_22363_pilon_pilon-exonerate_est2genome-gene−0.0-mRNA-1	Proteasome subunit beta5i	PB	T1	Schistosoma japonicum	1.40e−120
maker-contig_24278_pilon_pilon-snap-gene−0.33-mRNA-1	Calpain	CA	C2	Schistosoma margrebowiei	1.80e−154
maker-contig_28754_pilon_pilon-snap-gene−0.18-mRNA-1	Proteasome alpha subunit G1	PB	T1	Schistosoma mansoni	3.40e−110
maker-contig_46585_pilon_pilon-augustus-gene−0.2-mRNA-1	Proteasome subunit alpha 1	PB	T1	Clonorchis sinensis	1.70e−130
maker-contig_4765_pilon_pilon-exonerate_est2genome-gene−0.1-mRNA-1	Proteasome alpha subunit G1	PB	T1	Schistosoma mansoni	2.90e−105
maker-contig_6883_pilon_pilon-snap-gene−0.23-mRNA-1	Cytosol alanyl aminopeptidase	MA	M1	Echinococcus granulosus	1.30e−138
maker-contig_7443_pilon_pilon-snap-gene−0.43-mRNA-1	Proteasome subunit alpha 6	PB	T1	Schistosoma japonicum	2.70e−62

^*Indicates ether hydrolases with peptidase activity included in MEROPS database. Peptidase clan. MA: includes different families with aminopeptidase (M1, M16), carboxypeptidase (M2, M32), peptidyl-dipeptidase (M2), oligopeptidase (M3, M13) and endopeptidase activity (M4, M10, M12). MF: Presents solely proteins belonging to the M17 family, which have a predominant aminopeptidase activity. PB: Autocatalytic activation peptidase activity except for family T1. CA: Presents proteins with mostly endopeptidase activities; however, proteins belonging to C1 family present a predominant exopeptidase activity. Peptidase family. M1: Peptidase family mainly containing metallo-catalytic aminopeptidases dependent on a single zinc ion. M17: Peptidase family containing metallo-catalytic aminopeptidases with co-catalytic metal ions. T1: Peptidase family containing the peptidases components of proteasomes and related compound peptidases holding a threonine catalytic site. C2: Peptidase family containing endopeptidases termed “calpains” which hold a cysteine catalytic site

ISSN: 1756-3305