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Fig. 2 | Parasites & Vectors

Fig. 2

From: Echinococcus granulosus ubiquitin-conjugating enzymes (E2D2 and E2N) promote the formation of liver fibrosis in TGFβ1-induced LX-2 cells

Fig. 2

Expression, purification, and Western blotting of rEgE2D2 and rEgE2N. M: Protein marker; (1) Protein of pET32a (+); (2) rEgE2D2/rEgE2N crude protein; (3) Purified rEgE2D2 and rEgE2N; (4) Purified pET32a (+) protein; (5) Purified rEgE2D2-IgG and rEgE2N-IgG; (6) Purified recombinant protein probed with rat polyclonal antibody; (7) Purified recombinant protein probed with pre-immunized rat serum; (8) Purified recombinant protein probed with serum from sheep naturally infected with E. granulosus; (9) Purified recombinant protein probed with non-infected sheep serum; (10) Total protein of PSCs probed with rat polyclonal antibody; (11) Total protein of PSCs probed with pre-immunized rat serum

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