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Table 1 Primers used for the detection of knockdown resistance mutations in house flies in Abu Dhabi, United Arab Emirates

From: kdr mutations and deltamethrin resistance in house flies in Abu Dhabi, UAE

Primer

Direction

Sequence 5′-3′

Primer size (bp)

Annealing temperature (°C)

Used for

References

kdr 1

Forward

AAGGATCGCTTCAAGG

16

54

PASA

[34]

kdr 2

Reverse

GTCGTGATCGGCAATT

16

54

PASA

[34]

kdr 3

Forward

CGTCAACTTACCACAAG

17

54

PASA

[34]

kdr 4

Reverse

TTCACCCAGTTCTTAAAACGAG

22

54

PASA

[34]

K1

Forward

TCGCTTCAAGGACCATGAAT

20

60

Sequencing

[47]

K2

Reverse

TTACGTTTCACCCAGTTCTTA

21

60

Sequencing

[47]

4Mut_kdr_F

Forward

TCCGGAATTGGAGAAGGTGC

20

55

Sequencing

This study

4Mut_kdr_R

Reverse

TCAAGCCATCGCCCATGATT

20

55

Sequencing

This study

  1. kdr Knockdown resistance, PASA PCR amplification of specific alleles