Fig. 5
From: kdr mutations and deltamethrin resistance in house flies in Abu Dhabi, UAE
Genotyping of the house fly Musca domestica based on the kdr mutation L1014F using DNA sequencing. Sequences are aligned with the genomic reference sequence (GenBank accession number NW_026712250.1) using Unipro UGENE software. The chromatograms were viewed using SnapGene software (www.snapgene.com). The mutation occurs at position number 125581. Susceptible (SS) insects have the sequence CTT; heterozygous (RS) insects have the sequence YTT, where Y is C/T; and resistant homozygous (RR) insects have the sequence TTT. Chromatograms show a single peak in SS and RR genotypes and two peaks in RS genotypes at the kdr mutation position. kdr, Knockdown resistance; RR, homozygous resistant (kdr/kdr); RS, heterozygous (kdr/sus); SS, homozygous susceptible (sus/sus)