Fig. 8
![Fig. 8](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13071-023-06105-4/MediaObjects/13071_2023_6105_Fig8_HTML.jpg)
In vitro mixing of red and green fluorescent epimastigotes; 1/148 RFP and Gam2 GFP epimastigotes were mixed in vitro, but very few groups of red and green trypanosomes such as that shown in A were found, most clumps being a single colour. Co-cultures were found to contain a few yellow fluorescent trypanosomes (arrowed) at 21 days post-mixing (B–D), presumably resulting from cytoplasmic exchange between cells of the different strains. From left to right: phase contrast, green fluorescence, red fluorescence, merge of red and green fluorescence. A Scale bar = 20 µm; B–D scale bar = 10 µm