Fig. 6

A–C EgCF induces higher H3K27me3 levels at the promoters of NLRP3 and IL-1β. Chromatin immunoprecipitation was performed in PM and THP-1 cells treated with EgCF and LPS for 4 h. qRT-PCR was used to detect the enrichment of H3K27me3 modification at NLRP3 and IL-1β promoters (left). The amplified products were visualized by agarose gel electrophoresis (right). Data are presented as mean + SEM of three replicates, representing one of three independent experiments, and compared using one-way ANOVA and Tukey’s test; * P < 0.05, ** P < 0.01, *** P < 0.001. For abbreviations, see Figs. 1,  3 and 5