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Fig. 3 | Parasites & Vectors

Fig. 3

From: Plasmodium vivax merozoite-specific thrombospondin-related anonymous protein (PvMTRAP) interacts with human CD36, suggesting a novel ligand–receptor interaction for reticulocyte invasion

Fig. 3

CD36 is selectively abundant on the surfaces of reticulocytes. A Dot plot diagrams showing the unstained RBCs (left) and RBCs single stained with thiazole orange (TO) (center) for gating. The application of this gating strategy to RBC dual staining with TO and anti-human CD36 antibody is indicated in the right diagram. Numbers in quadrants represent gate frequencies. B CD36 was detected on 19.34 ± 2.14% of reticulocytes [TO (+)] but mostly disappeared during the maturation of RBCs (0.30 ± 0.09% of normocytes [TO (−)] were positive for CD36). Among the reticulocyte-enriched RBCs used for the downstream binding assay (total), 11.35 ± 3.23% of cells expressed CD36. The bars represent the mean ± SEM from three biological replicates. Single asterisks, P < 0.05. C Histogram of the fluorescence signal from a single stain with a mouse anti-human CD36 antibody (red) and its isotype control (blue)

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Parasites & Vectors

ISSN: 1756-3305

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