Table 1 Primers used to amplify Borrelia genes in this study
From: Borrelia puertoricensis in opossums (Didelphis marsupialis) from Colombia
Gene | Round | Primer name | Secuencia 5′–3′ | Tm [°C] | bp |
|---|---|---|---|---|---|
16S rRNA qPCR | Screening | Bor16S3F | AGCCTTTAAAGCTTCGCTTGTAG | 60 | 148 |
Bor16S3R | GCCTCCCGTAGGAGTCTGG | ||||
probe Bor16S3P | [6FAM] CCGGCCTGAGAGGGTGAACGG | ||||
16S rRNA | First round | FD3 [f] | AGAGTTTGATCCTGGCTTAG | 54 | 1489 |
T50 [r] | GTTACGACTTCACCCTCCT | ||||
Second round | FD3 [f] | AGAGTTTGATCCTGGCTTAG | 56 | 730 | |
16 s-1 [r] | TAGAAGTTCGCCTTCGCCTCTG | ||||
Second round | 16 s-2 [f] | TACAGGTGCTGCATGGTTGTCG | 56 | 462 | |
T50 [r] | GTTACGACTTCACCCTCCT | ||||
Second round | Rec4 [f] | ATGCTAGAAACTGCATGA | 54 | 520 | |
Rec9 [r] | TCGTCTGAGTCCCCATCT | ||||
flaB (flagellin) | First round | FlaRL [f] | GCAATCATAGCCATTGCAGATTGT | 55 | 665 |
FlaLL [r] | ACATATTCAGATGCAGACAGAGGT | ||||
Second round | FLaRS [f] | CTTTGATCACTTATCATTCTAATAGC | 55 | 491 | |
FlaLL [r] | ACATATTCAGATGCAGACAGAGGT | ||||
Second round | FlaRL [f] | GCAATCATAGCCATTGCAGATTGT | 55 | 528 | |
FLaLS [r] | AACAGCTGAAGAGCTTGGAATG |