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Table 1 Parasite detection rates from sugar-soaked feeding substrates, fed on by an individual mosquito or by groups of mosquitoes

From: Testing a non-destructive assay to track Plasmodium sporozoites in mosquitoes over time

 

P. berghei (days 23, 25 pIBM)

P. chabaudi (days 13, 15 pIBM)

Detection ratea

Ctb

Sporozoitesb

Det. ratea

Ctb

Sporozoitesb

Individual mosquitoes

First collected substrate

0.25 (3/12)

33.6 ± 0.6

47.4 ± 16.5

0.11 (1/9)

37.9

1.5

Second collected substrate

0.33 (4/12)

35.2 ± 0.5

13.3 ± 4.0

0.22 (2/9)

37.5 ± 0.8

2.2 ± 1.1

Groups of mosquitoes

First collected substrate

0.40 (2/5)

33.0 ± 2.3

172 ± 160

n.d

n.d

n.d

Second collected substrate

0.60 (3/5)

34.6 ± 1.2

34.4 ± 21.8

n.d

n.d

n.d

  1. Ct Cycle threshold, n.d not done, pIBM post-infectious blood meal
  2. aProportion of positive substrates (number positive substrates/total tested substrates)
  3. bMean (± standard error of the mean [SEM]) Ct value or mean (± SEM) number of sporozoites per PCR for positive substrates
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Parasites & Vectors

ISSN: 1756-3305

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