Fig. 4
From: Testing a non-destructive assay to track Plasmodium sporozoites in mosquitoes over time
Linearity of quantification for P. berghei (orange; a) and P. chabaudi (blue; b) reference DNA (cotton wool, 8% fructose). Mean Ct values ± SEM are presented for the reference DNA originating from infected mosquitoes (log10 genomes/PCR, mosquito), ranging from 17 to 3461 genomes/PCR (P. berghei) or from 3 to 5949 genomes/PCR (P. chabaudi) (n = 3/concentration/species). The LOQ (Ct at which samples can be detected but quantification becomes inaccurate) is determined as the switch point where the plateau ends. The dashed line shows the Ct value associated with the LOD ± SEM (see Fig. 2) for blood samples. Ct, Cycle threshold; LOD limit of detection; LOQ, limit of quantification; Pb, Plasmodium berghei; Pc, Plasmodium chabaudi; SEM, standard error of the mean