Genotypea | Origin |
|---|
ITS | 18S | cox1 mitotype | Genotypea | Laboratory/field isolate | Country | Life-cycle stage | n |
|---|
Sh | Sh | Sh | Sh | Laboratory | Egypt | Adult | 3 |
Sh | Sh | Sh | Sh | Field | Malic | Miracidia | 58 |
Sb | Sb | Sb | Sb | Laboratory | Spain | Adult | 3 |
Sb | Sb | Sb | Sb | Field | Mali | Adult | 37 |
Sc | Sc | Sc | Sc | Field | Mali | Adult | 31 |
Sc | Sc | Sc | Sc | Field | Senegal | Adult | 12 |
Sh and (Sb or Scb) | Sh and Sb | Sb | Hybrid Sh/Sb | Laboratory F1 | Egypt/Spain | Adult | 3 |
Sh and (Sc or Sbb) | Sh and Sc | Sh | Hybrid Sh/Sc | Field | Malic | Miracidia | 19 |
Sh and (Sc or Sbb) | Sh and Sc | Sh | Hybrid Sh/Sc | Field | Ivory Coastd | Miracidia | 3 |
- cox1, Cytochrome oxidase subunit 1 mitochondrial gene, ITS internal transcribed spacer, n number of individuals, Sb Schistosoma bovis, Sc Schistosoma curassoni, Sh Schistosoma haematobium, SNP single nucleotide polymorphism, T–ARMS tetra-primer amplification refractory mutation system
- aThe genetic profiles are inferred by Sanger sequencing of the T-ARMS regions of the ITS, 18S and by the rapid diagnostic multiplex PCR (RD-PCR) region of the cox1 DNA [45]
- bThe sequence of the ITS or 18S cannot be distinguished between the two species as the region analysed does not contain species-specific SNPs
- cEthical clearance was obtained from the Faculty of Medicine, Pharmacology, and Odonto-Stomatology of Mali (reference no.2018/71/CE/FMPOS)
- dEthical clearance was obtained from the Ministry of Health and Public Hygiene in Ivory Coast (reference no.003–18/MSHP/CNER-kp) [38]
