Fig. 5From: Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding systemDetermination of the minimum dose of T. parva sporozoites from the in vitro tick feeding system sufficient to infect bovine lymphocytes in vitro. Lymphocytes were probed with anti-PIM monoclonal antibody. a Unstimulated and ConA-stimulated negative controls. b Flow cytometric detection of infected lymphocytes exposed to tenfold serial dilutions of T. parva sporozoites isolated from the in vitro tick feeding systemBack to article page