Fig. 2

Alignment of the domain IS6 and partial domain I–II linker fragment of the vgsc gene of C. hemipterus with Accession No. MK908976 against the reference vgsc gene of C. lectularius wild type with Accession No. FJ031996. The mutation sites are indicated in vertical boxes; orange, no point mutation at V419 site, green, silent mutation with transition at A454 and K458 sites, and red, missense mutation with transition at A468T site. Nucleotides 1–147 include exon I, 148–211 intron I, and 212–355 exon II. NS and AS represent nucleotide sequence and deduced amino acid sequences, respectively. The location of primers are indicated with red arrows