Fig. 2

Identification of T. cruzi TBP and SNAP50 proteins in the protein complex specifically bound to the Pr77 sequence. a Dynabeads protein capture assay coupled to western blotting was performed by incubating 1.5 mg of T. cruzi total protein extract with 80 pmol of the biotinylated double-stranded DNA probes L1TcPr77, T. cruzi SL and human IL6. Beads without a DNA probe (No DNA) were used as a negative control. Flow-through (FT) and eluted (E) fractions following benzonase treatment were loaded into a 10% SDS-polyacrylamide gel and transferred to nylon membranes for western blotting analyses employing a anti-TBP and b anti-SNAP50 specific antibodies. Ten micrograms of total protein extract (TP) was used as a positive control. Detected bands are labelled by a black arrowhead. Molecular weight marker (MW) is shown in kDa