Fig. 4

Downregulation of lncRNA028466 promotes naive CD4⁺T cell differentiation toward the Th1 phenotype. a The interference efficiency of siRNAs was tested by qRT-PCR. b The mRNA expression of cytokines in the transfected naive CD4⁺T cells was tested by qRT-PCR. c The protein levels of cytokines were determined by western blot. d The supernatants were detected by ELISA. e The differentiation of the transfected naive CD4⁺T cells was measured by FACS. The scattered dots in the box represent the expression of IFN-γ and IL-2 producing Th1 cells, and IL-4, and IL-10 producing Th2 cells. The data came from a single experiment and represented three separate experiments. Negative represented the control group, siRNA1 represented the lncRNA028466 knockdown group. Data were expressed as mean ± SD and represented three separate experiments (n = 6 per group). *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant