Fig. 3

Overexpression of lncRNA028466 biases naive CD4⁺T cell differentiation toward the Th2 phenotype. a Validation of the transfection efficiency of lncRNA028466 overexpression lentivirus by qRT-PCR and representative image of the expression of GFP (×40) was detected by a laser scanning confocal microscope (Olympus). b The mRNA expression of IFN-γ, IL-2, IL-4, and IL-10 was measured by qRT-PCR. c The protein levels of cytokines were determined by western blot. d The supernatants were detected by ELISA. e The differentiation of the transfected naive CD4⁺T cells was measured by FACS. The scattered dots in the box represent the expression of IFN-γ and IL-2 producing Th1 cells, and IL-4 and IL-10 producing Th2 cells. Results are shown as mean ± SD and represent three separate experiments (n = 6 per group). Vector represents empty vector, pCDH-028466 represents lncRNA028466 overexpression vector. *P < 0.05, **P < 0.01, ***P < 0.001