The Eagle effect in the Wolbachia-worm symbiosis

Parasites & Vectors

Table 1 Viability of female worms treated with doxycycline was highly correlated with worm motility

	% Inhibition of motility					% Inhibition of formazan production
Compound	Day 1 (%)	Day 2 (%)	Day 3 (%)	Day 4 (%)	Day 6 (%)	Day 6 (%)
Doxycycline (100 µM)	54	94	97	99	99	91
Doxycycline (10 µM)	0	0	9	59	92	46
Doxycycline (1 µM)	0	0	0	2	0	8

Worms treated with 100 and 10 µM doxycycline showed declining motility over time and were barely motile at 100 µM by Day 2. Viability was assessed on Day 6, as measured by formazan production in an MTT assay. The degree and duration of motility inhibition was predictive of reduced viability in the MTT assay

ISSN: 1756-3305