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Table 1 Analysed sequences of A. phagocytophilum in this herd in combination with clinical symptoms of the infected animals

From: Epidemiology, genetic variants and clinical course of natural infections with Anaplasma phagocytophilum in a dairy cattle herd

Cow no./ tick no./roe deer no. Date of positive PCR Ct-value Clinic 16S rRNA groEL msp2 msp4
7 19.06.11 23 not examined 16S-20 (W) g-18 (X) M2-26 M4-49
14 04.06.11 17 F, RM 16S-20 (W) g-18 (X) M2-26 M4-49
22 30.05.11 15 F, RM, LLO 16S-20 (W) g-18 (X) M2-26 M4-49
  09.07.11 28.5 no clinic 16S-20 (W) too short negative M4-50
  03.10.11 28 no clinic 16S-20 (W) g-15 (N) negative M4-51
28 03.10.11 20 F, RM, DC 16S-20 (W) g-15 (N) negative M4-51
30 17.05.11 27.5 F, RM, DC 16S-20 (W) g-18 (X) too short M4-50
35 17.05.11 15 F, RM 16S-20 (W) g-18 (X) M2-26 M4-49
36 19.05.11 13.5 F, RM, DC 16S-20 (W) too short M2-26 M4-49
42 04.09.11 36 not examined 16S-20 (W) too short negative M4-49
46 22.05.11 19.5 F, RM, DC, LLO 16S-20 (W) g-18 (X) M2-26 M4-49
  21.08.11 30 no clinic negative negative negative M4-50
49 19.05.11 31.5 F, RM, DC, LLO 16S-20 (W) g-15 (N) negative M4-51
52 14.09.11 19.5 F, RM, LLO 16S-20 (W) too short M2-27 M4-51
53 22.06.11 14 F, RM, DC 16S-20 (W) g-18 (X) negative M4-49
57 04.06.11 18.5 F, RM, DC 16S-20 (W) g-18 (X) M2-26 M4-49
  04.09.11 30 no clinic 16S-20 (W) negative negative M4-49
58 17.05.11 24.5 F, RM, DC 16S-20 (W) g-18 (X) M2-26 M4-49
  09.07.11 31 no clinic 16S-20 (W) negative negative negative
59 04.09.11 26.5 no clinic 16S-22 (Y) too short negative M4-13 (n)
61 22.06.11 18 F, RM, DC, LLO 16S-20 (W) g-18 (X) M2-26 M4-49
  09.07.11 22 no clinic 16S-20 (W) negative M2-27 M4-51
Tick 83a 13.05.11 29   16S-7 (I) negative negative negative
Tick 90b 06.06.11 20   16S-20 (W) too short negative M4-51
Tick 94b 06.06.11 23   16S-20 (W) too short negative M4-49
Tick 96b 06.06.11 19   16S-20 (W) too short negative M4-51
Tick 167a 25.06.11 30   16S-22 (Y) too short negative M4-13 (n)
Roe deer 1 26.06.11 30   16S-21 (X) negative M2-9 (J) too short
Roe deer 2 17.09.11 27.5   16S-21 (X) negative negative negative
Roe deer 3 15.10.11 30   16S-19 (V)-like sequence too short negative too short
  1. Abbreviations: F fever, RM reduced milk yield, DC discharge from eyes and nose, LLO lower limb oedema and stiff walking
  2. Note: Nomenclature of gene variants is following previous denominations [17, 23, 25] with numbers after the gene abbreviation. The letter codes shown for some of these variants are an alternative, also unofficial nomenclature. Particular combinations of these four gene variants were frequently observed with 16S-20 (W), g-18 (X), M2-26, and M4-49 are given in bold; negative = gene locus could not be amplified; too short = gene locus was amplified but the sequence read was too short for full comparison and thus for allocation to a particular variant. Ct-values are provided as mean of two independent real-time PCRs targeting the msp2-gene, which was used for screening. Please note that tick screening was done only once. A ct-value ≤38 was considered positive for A. phagocytophilum DNA
  3. aCollected from roe deer
  4. bCollected from heifer