A proteomic insight into the midgut proteome of Ornithodoros moubata females reveals novel information on blood digestion in argasid ticks

Oleaga, Ana; Obolo-Mvoulouga, Prosper; Manzano-Román, Raúl; Pérez-Sánchez, Ricardo

doi:10.1186/s13071-017-2300-8

Table 7 Proteins involved in the stress responses associated to blood digestion that were identified in the midgut of Ornithodoros moubata females before feeding (unfed) and at 48 h post-feeding (fed). (T) Gene identified in the intestinal transcriptome of O. moubata [29]

From: A proteomic insight into the midgut proteome of Ornithodoros moubata females reveals novel information on blood digestion in argasid ticks

Experimental group	Database	UniProt ID	Protein	emPAI Unfed	emPAI Fed	Molecular function
Antioxidants
Unfed	EST_Acari	B7Q8W6	Alkyl hydroperoxide reductase, thiol specific antioxidant	0.53	–	Oxidoreductase activity
Unfed	mRNA_Om	B7QGB0	Peroxidase (T)	0.09	–
Unfed	mRNA_Om	B7PUM7	Peroxinectin (T)	0.1	–
Unfed	NCBI_metazoa	Q9GV35	Peroxiredoxin (T)	0.46	–
Unfed	EST_Acari	B7QN17	Thioredoxin-dependent peroxide reductase (T)	0.99	–
Fed	EST_Acari	T2FDK5	Catalase (T)	–	1.45
Fed	EST_Acari	A6N9S1	Thioredoxin peroxidase (T)	–	1.84
Unfed-Fed	EST_Acari	F0JAD0	Dihydrolipoamide dehydrogenase	2.44	0.32
Unfed-Fed	EST_Acari	Q2XW17	Glutathione peroxidase (T)	1.65	1.1
Unfed-Fed	EST_Acari	B7PUC4	Superoxide dismutase [Cu-Zn] (T)	4.58	2.15
Unfed-Fed	mRNA_Om	E2ASR9	Thioredoxin-like protein 1 (T)	1.68	0.17
Unfed-Fed	EST_Acari	A6NA14	Truncated peroxiredoxin	1.04	0.61
Unfed-Fed	mRNA_Om	B7PTG8	Thioredoxin reductase (T)	0.53	0.04
Chaperones
Unfed	EST_Acari	B7Q6Y2	Chaperonin subunit	0.58	–	Protein folding
Unfed	EST_Acari	A0A097A1J8	Heat shock 70 kDa protein	18.61	–
Unfed	NCBInr	gi\|557,767,195	heat shock 70 kDa protein cognate 4-like	0.2	–
Unfed	NCBI_metazoa	Q4ZJ79	Heat shock cognate 70 protein (T)	0.2	–
Unfed	EST_Acari	B7PEU9	Heat shock protein (T)	0.35	–
Unfed	EST_Acari	B7PIN1	Heat shock protein 20.6 (T)	0.27	–
Unfed	NCBI_metazoa	Q5I5Q6	Heat shock protein 60 (T)	0.23	–
Unfed	EST_Acari	B7QI01	Hsp90 protein (T)	2.82	–
Unfed	mRNA_Om	G3MRN7	Putative uncharacterized protein	0.27	–
Unfed	EST_Acari	B7P3Z6	T-complex protein 1 subunit gamma (T)	1.06	–
Unfed	NCBInr	E4WZX2	Whole genome shotgun assembly, reference scaffold set	0.08	–
Fed	EST_acari	F0 J987	Endoplasmic reticulum glucose-regulated protein	–	1.8
Fed	mRNA_Om	B4JT04	GH23301	–	0.17
Fed	EST_acari	E4W3Z2	Heat shock 70 kDa protein 5 (T)	–	8.27
Fed	NCBInr	B4YTT9	Heat shock protein 70–2 (T)	–	0.19
Fed	EST_Acari	F0J8P3	HSP70 family member (T)	–	7.42
Fed	mRNA_Om	B7Q150	Molecular chaperone	–	0.1
Fed	mRNA_Om	G3MQW7	Putative uncharacterized protein	–	0.21
Fed	mRNA_Om	B7PN00	TPR domain-containing protein (T)	–	0.11
Fed	NCBI_metazoa	U4TVI5	Uncharacterized protein	–	0.1
Unfed-Fed	EST_Acari	B7PGQ2	Calnexin	0.51	0.67
Unfed-Fed	EST_Acari	Q68HD1	Calreticulin	1.4	0.73
Unfed-Fed	EST_Acari	B7PZ24	Chaperonin complex component, TCP-1 delta subunit	0.27	0.23
Unfed-Fed	EST_Acari	B7QJ21	Chaperonin complex component, TCP-1 eta subunit	0.23	1.67
Unfed-Fed	mRNA_Om	G6DGE9	Chaperonin containing t-complex polypeptide 1 beta subunit	4.89	6.06
Unfed-Fed	EST_Acari	B7PEV0	Chaperonin subunit	1.11	1.04
Unfed-Fed	EST_Acari	F0J8S6	FKBP-type peptidyl-prolyl cis-trans isomerase	0.44	0.26
Unfed-Fed	mRNA_Om	B4KRE5	GI20465	1.34	0.91
Unfed-Fed	EST_Acari	A0A087TSI6	GrpE protein homolog	0.36	0.85
Unfed-Fed	mRNA_Om	B7PPJ5	Grpe protein	0.34	0.47
Unfed-Fed	EST_Acari	B7PWF5	Heat shock protein 20.5 (T)	3.55	0.47
Unfed-Fed	mRNA_Om	B7Q0B3	Heat shock protein 70 (HSP70)-interacting protein (T)	0.59	0.54
Unfed-Fed	NCBI_metazoa	M9WB33	Heat shock protein 90	0.21	3.51
Unfed-Fed	EST_Acari	F0J8Z3	Mitochondrial chaperonin Cpn60/Hsp60p	2.85	1.16
Unfed-Fed	EST_Acari	B7PD56	Peptidyl-prolyl cis-trans isomerase (T)	4.59	0.66
Unfed-Fed	mRNA_Om	G3MKV9	Putative uncharacterized protein	1.37	1.42
Unfed-Fed	EST_Acari	A0A087UTZ9	T-complex protein 1 subunit alpha (T)	0.71	0.97
Unfed-Fed	mRNA_Om	B7PRH5	T-complex protein 1 subunit delta (T)	2.04	4.61
Unfed-Fed	EST_Acari	B7QC85	Tumor rejection antigen (Gp96)	1.29	1.32
Unfed-Fed	mRNA_Om	H9JT91	Uncharacterized protein (T)	2.64	6.15
Detoxification
Unfed	mRNA_Om	Q9Y1T8	Cytochrome P450 4 W1 (T)	0.37	–	Heme binding
Unfed	mRNA_Om	B7P9Q4	Cytochrome P450 (T)	0.05	–
Fed	EST_Acari	B7P5J0	Cytochrome P450 (T)	–	0.74
Fed	mRNA_Om	B7QES0	Cytochrome P450 (T)	–	1.18
Fed	mRNA_Om	B7QJS5	Cytochrome P450 (T)	–	0.23
Unfed-Fed	mRNA_Om	B7PME9	Cytochrome P450 (T)	0.98	1.78
Unfed-Fed	mRNA_Om	B7PG84	Glutathione S-transferase (T)	21.84	2.09	Transferase activity
Unfed-Fed	EST_Acari	B7PR98	Glutathione S-transferase (T)	4.24	4.97
Unfed-Fed	mRNA_Om	B7Q917	Glutathione S-transferase (T)	0.8	0.69
Unfed-Fed	mRNA_Om	B7PRA0	Glutathione S-transferase (T)	2.54	0.33
Unfed-Fed	mRNA_Om	B7QMI2	Glutathione S-transferase (T)	9.09	0.49

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