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Table 4 Accuracy and precision of the detection of NATOG using UPLC-Fluorescence (n = 8)

From: Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,β-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection

 

QC-Low (2 μM)

QC-Medium (10 μM)

QC-High (50 μM)

Milli-Q Water

   

 Mean

2.03

10.2

52.3

 Standard deviation

0.02

0.34

1.94

 Coefficient of variation (%)

1.0

3.3

3.7

 % Accuracy

102

102

105

Human urine

   

 Mean

2.31

10.3

52.2

 Standard deviation

0.08

0.17

0.56

 Coefficient of variation (%)

3.5

1.7

1.1

 % Accuracya

115

103

104

  1. aEndogenous level of NATOG used for preparation of QC samples was 0.31 μM; results have not been corrected for the endogenous levels