Fig. 2
Expression of the RmAQP2 gene in unfed larvae, engorged nymphs, unfed males, and salivary glands, gut and ovaries of partially engorged (day 5 of feeding) R. microplus females. Six biological replicates of unfed larvae (approximately 100 larvae per sample), engorged nymphs (approximately 10 nymphs per sample), unfed males (approximately 10 males per sample), and individual salivary glands, ovaries and guts of partially engorged females (at day 5 of feeding) were analyzed by reverse transcriptase quantitative real-time PCR. The transcription level of RmAQP2 was calculated as relative quantity using the delta C t comparative method normalized by the total amount of RNA used to generate the cDNA. Different letters (a, b) above the bars indicate significant statistical differences (one-way ANOVA, Tukey post hoc test, P < 0.001)