Fig. 2

The alteration of mouse retina and ganglion cells before and after treatment regimen following infection with A. cantonensis. a1-f1: retina in different treatment groups with HE staning. bar = 100 μm.↑ points ganglion cell layer of retina. a2-f2: Electron microscopy images of ganglion cells in different treatment groups. ↓ points cytoplasm of ganglion cells; ▲ points the nucleusof ganglion cells. bar = 1 μm. a1-a2: retina and ganglion cell in normal group. There was a layer of ganglion cells (↑ in A1). The cytoplasm and nucleus of ganglion cells were intact (↓ and ▲ in A2). b1-b2: retina and ganglion cell in groups infected with A. cantonensis at 21 dpi. The ganglion cell layer showed inflammatory cell infiltration (↑ in B1). Swelling was pronounced in cytoplasm of ganglion cells (↓in B2) and nucleus diminished. c1-c2: Albendazole (AB) treatment for 7 days after 14 dpi with A. cantonensis. Inflammation was alleviated in ganglion cell layer of retina (↑ in C1). Swelling can still be observed in cytoplasm of ganglion cell (↓ in C2) with an intact nuclear as usual (▲ in C2). d1-d2: Albendazole combined with Dexamethasone treatment (AB + De) for 7 days after 14 dpi with A. cantonensis. The inflammation of the retina almost resolved (↑ in D2). Swelling of ganglion cytoplasm relieved (↓ in C2). The nucleus was still obviously observed (▲ in D2). e1-e2: Albendazole combined with CPT (AB + CPT)treatment for 7 days after 14 dpi with A. cantonensis. Inflammation was alleviated in ganglion cell layer of retina (↑ in E1). Swelling decreased greatly in cytoplasm of ganglion cell (↓ in E2). f1-f2: Albendazole combined with TSII-A (AB + TSII-A) treatment for 7 days after 14 dpi with A. cantonensis. Inflammation was alleviated slightly in ganglion cell layer of retina (↑ in F1). Swelling diminished in cytoplasm of ganglion cell (↓ in F2) and the cells regained normal structure as in A2. g: Retinal thickness measured under a light microscope in normal group, 21 dpi infection group, albendazole treatment group (AB), albendazole combined with dexamethasone (AB + De) treatment group, albendazole combined with CPT (AB + CPT) treatment and albendazole combined with TSII-A (AB + TSII-A) treatment group. Data are mean ± SEM. *Statistically significant when compared with control (0 dpi) (P < 0.05); # Statistically significant when compared with 21 dpi (P < 0.05)