Fig. 7
The Viral Titration of Vero Cell Infected with CHIKV Evaluated by RTCA. Measuring cell impedance every 2 minutes by RTCA monitored the viral dose-dependency effect of various dilutions of CHIKV inoculum on the proliferation, spreading and adhesion of cells. The black vertical line indicates the normalisation of the CI time point at 26 h subsequent to CHIKV infection of the Vero cell line. Coloured curves represent the Vero cells infected with different concentrations of CHIKV. Blue line: 10−1 CHIKV; Pink line: 10−2 CHIKV; Turquoise line: 10−3 CHIKV; Purple line: 10−4 CHIKV; Grey line: 10−5 CHIKV and Orange line: 10−6 CHIKV; Green line: negative control (without CHIKV infection). Each data point signifies the average ± standard deviation and was analysed in triplicate