Fig. 2

The Effect of the Intensified Concentrations of Ribavirin on Vero Cells Evaluated by both RTCA and MTS Assays. a The cell proliferation after 138 h of incubation with increasing concentrations of ribavirin. The black vertical line indicates the normalisation of the CI time point at 26 h subsequent to Vero cells being treated with ribavirin. Coloured curves represent the various serial dilutions of ribavirin. Each data point signifies the average ± standard deviation and was analysed in triplicate. Blue line: 200 μg/ml ribavirin; Pink line: 100 μg/ml ribavirin; Turquoise line: 50 μg/ml ribavirin; Purple line: 25 μg/ml ribavirin; Grey line: 12.5 μg/ml ribavirin; Green line: negative control (without ribavirin). Each data point signifies the average ± standard deviation and was analysed in triplicate. b In comparison to the RTCA assay, this figure indicates that the Vero cells were viable even when treated at the highest concentration of ribavirin on both days two and three, showing minimal cytotoxic effects of the compound on the cells via MTS assay