Molecular markers for analyses of intraspecific genetic diversity in the Asian Tiger mosquito, Aedes albopictus

Manni, Mosè; Gomulski, Ludvik M; Aketarawong, Nidchaya; Tait, Gabriella; Scolari, Francesca; Somboon, Pradya; Guglielmino, Carmela R; Malacrida, Anna R; Gasperi, Giuliano

doi:10.1186/s13071-015-0794-5

Parasites & Vectors

Table 6 The 23 Aedes albopictus microsatellite loci validated in this study

From: Molecular markers for analyses of intraspecific genetic diversity in the Asian Tiger mosquito, Aedes albopictus

Locus	Type ¹	Repeat motif	Primer sequences (5’ to 3’) ²	T _a (°C) ³
Aealbmic1	P	(TC)₁₀	F: [6FAM]ACCTTGCGTAGGATGACGAT	60
Aealbmic1	P	(TC)₁₀	R: GCCATGATCACGAGCCTATT	60
Aealbmic2	I	(GTT)₅TGGAGTGAG(GTT)₆	F: [6FAM]ACGATGCGTAACCATTCGAT	58
Aealbmic2	I	(GTT)₅TGGAGTGAG(GTT)₆	R: AACACCGCCGAATATGAAAC	58
Aealbmic3	P	(AAC)₉	F: [HEX]ACCATACAGCCTGGAGTTCG	58
Aealbmic3	P	(AAC)₉	R: GGGGTTGTGTGAATTGTCGT	58
Aealbmic4	P	(CAA)₈	F: [6FAM]ATCGCGGGTTTTCTATTCCT	58
Aealbmic4	P	(CAA)₈	R: ATCAACGAAACCGAAAGCAT	58
Aealbmic5	P	(TGT)₁₃	F: [HEX]AACCCATCGAACACAGAAGG	58
Aealbmic5	P	(TGT)₁₃	R: GTACGGTTGACTCGCTGTGA	58
Aealbmic6	I	(GTT)₃GCT(GTT)₃ GGT(GTT)₄	F: [HEX]GATGGTCCGTATTTGGGTTG	57
Aealbmic6	I	(GTT)₃GCT(GTT)₃ GGT(GTT)₄	R: ATCTTCACTCATCCGCCATC	57
Aealbmic7	I	(TTG)₈ATG(TTG)₄	F: [HEX]ATAGACGGGAGTCGGTTCCT	58
Aealbmic7	I	(TTG)₈ATG(TTG)₄	R: TCCAACCGCTAGTGTCATCA	58
Aealbmic8	P	(GT)₈	F: [HEX]TTGTTGTTCGGTTGTTGTTTG	57
Aealbmic8	P	(GT)₈	R: CGGGTTCCAACTATGTACGA	57
Aealbmic9	P	(GAT)₇	F: [HEX]GCGATGACAGTGGAACAAGA	58
Aealbmic9	P	(GAT)₇	R: GCTTGGCAGGGAACAAATTA	58
Aealbmic10	P	(ATC)₉	F: [6FAM]ATCGCCTTCACTCTTCTTCG	60
Aealbmic10	P	(ATC)₉	R: CCAATCCTGAGCCGTACATT	60
Aealbmic11	P	(TGT)₅	F: [6FAM]CTCTGCGTTCCGGTTCTATC	58
Aealbmic11	P	(TGT)₅	R: AGGCAACCTCTCGAATGAAA	58
Aealbmic12	P	(GAT)₇	F: [6FAM]AGAGCCCTCGAAAAGAGAGC	58
Aealbmic12	P	(GAT)₇	R: AGCACTCATTCTTGGCTTGG	58
Aealbmic13	I	(GAT)GAC(GAT)₄	F: [6FAM]TCACACCATGGTCAAAGCAT	58
Aealbmic13	I	(GAT)GAC(GAT)₄	R: TGCTGAGTTGAATGGAAACG	58
Aealbmic14	I	(CT)₁₁ T(CT)₂	F: [6FAM]CAGAAGGTCTTGGATTCACTC	58
Aealbmic14	I	(CT)₁₁ T(CT)₂	R: TTTCCAATTCGTTTCTGGTTC	58
Aealbmic15	P	(GTT)₇	F: [6FAM]GGAATGGTTCCCTGGCTAAT	58
Aealbmic15	P	(GTT)₇	R: CCAACTCCGAAGAAGCCATA	58
Aealbmic16	P	(CAT)₇	F: [HEX]CACAACAACGAGAGTGTCGAA	60
Aealbmic16	P	(CAT)₇	R: CCGAGGGCAACACGATATAC	60
Aealbmic17	P	(CAA)₁₅	F: TCACCACAACAAACGGAATC	58
Aealbmic17	P	(CAA)₁₅	R: AGAGTTTTGGGCGCAGTTTA	58
Aealbmic18 ⁴	I	(TG)₁₂TA(TG)₇	F: GGGCCGAATCTCATATACCA	59
Aealbmic18 ⁴	I	(TG)₁₂TA(TG)₇	R: CTCAAATCGGTTGACAGACG	59
Aealbmic19	P	(ATC)₉	F: ACATATAGGTAATCCTTGCGCTGAT	59
Aealbmic19	P	(ATC)₉	R: GTTCCCGTATTTGATATTTGCTTT	59
Aealbmic20	P	(CA)₄₄	F: GGAGGCCATCATAATTTCGAC	58
Aealbmic20	P	(CA)₄₄	R: AGTGGACCACAGACAGACCATT	58
Aealbmic21	P	(ATG)₇	F: CCCTACAGCCCTGATTGAGA	58
Aealbmic21	P	(ATG)₇	R: CGAGTTGGGATGTGTGATTG	58
Aealbmic22 ⁵	I	(AC)₄₅AT(AC)₂AT(AC)₅	F: AAATCGGTTGAGGTTTACTGC	59
Aealbmic22 ⁵	I	(AC)₄₅AT(AC)₂AT(AC)₅	R: ATTCTTAGCCGGCAACTACG	59
Aealbmic23	P	(ACA)₈	F: AACGGAGCGGAGTCGATTAT	58
Aealbmic23	P	(ACA)₈	R: CTACTACCCGCTGCCTTCTG	58

¹Motif type (P: perfect repeats; I: imperfect repeats).
²The fluorescent label (6FAM or HEX) on the forward primer is indicated for the 16 loci that were subsequently used for the population analysis.
³Annealing temperature (T _a).
^4,5SSR sequences that correspond to previously identified loci: (alb212) [57] and (AealbD2) [58], respectively.

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ISSN: 1756-3305

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