Table 1 Primers used to amplify gene fragments for dsRNA synthesis or for qRT-PCR analysis
Gene | Forward (F); Reverse (R) | dsRNA primers | qRT-PCR primers |
|---|---|---|---|
AAEL001033 | F | TTTCAAGCAACCGGTGACAG | ACACTTCGCTCATTCCAC |
R | TTGAGGGACGTTTTGGAAGC | ACCTTGCTGTCTCCATCC | |
AAEL001156 | F | GCAAAACTGACCATCCTGCA | CGATGTGGACTATACGGAAAC |
R | CCGTTCTTGCAGTTTCAGCT | GGATTACTTGACGGTGCTTC | |
AAEL001684 | F | CTGTGCCGGTTATTCAGCTC | GGTATTCGTCGGTGGTATCAG |
R | GGGATGTTGTTGATCGTCGG | GCCTCGTGTTCGGTTTCG | |
AAEL002084 | F | TTGCTGGACGAGAAGGAAGT | AAGGAGTACGAAGAGAAGAAG |
R | ACTGAGCTGGTTGGTGAAGA | CGGAAGCGGTTCATTAGG | |
AAEL002275 | F | AGATCGAGCACTGTTTCCGA | GTCGTTTGGTGCGGCGTTTG |
R | CGTATACATGGGCCCGATCT | CCTTGTTGTCCTCCTCATCCTTGG | |
AAEL003501 | F | CGCAAGGATCGGAAACCAAT | CAGCAGGATACGGTCTTC |
R | CATGCTGTAGATCGGGTTGC | GAATAGGTCGGATTGTTGG | |
AAEL003757 | F | AAAGGAGCGAAGGAGACCAA | GCGGCGGATGCGATTCTC |
R | AGGTAGTGTTTCAGGGCCTC | GACTCTTGGCGGAACGATAGC | |
AAEL004231 | F | AGCCAAAGGAAGTACGGTCA | GAGCATCAGTCGCACATC |
R | CTTTCAGCTCTCCGATGTGC | TCCTTCTCTCGCAGTAACC | |
AAEL004471 | F | CGCGCCAAGAAGAAGATCAA | AAGCACAACACCAGGAAC |
R | ATTTTGTCCCGCAGCATAGC | GAAACCATCAGCCAAAGC | |
AAEL004696 | F | GATACCGAAATGTGCACGCT | CCGTGCTTGAGTTGATAC |
R | CCGGTTCTTTGTCACTGCAA | ATTGGAATCTGATGGTGAG | |
AAEL004939 | F | TATCCTGGGCAGCTGAACTC | GAACACCACCGCCATCAC |
R | CAATGCGAGAAGGTACCGTG | CCTGCTTCTTCTTGACTTTCG | |
AAEL005010 | F | GTTTTCGTCGGTCCGGTTAG | CGGTGGAAGTGGATTGTC |
R | TTGGCTTGGGTCTCCTTGAT | CGTTCTGATTCTTGCTGATG | |
AAEL005975 | F | TGGACAAGGCGGAACAAAAG | GAGCAGAGATGGAGGAAC |
R | CTTGATTCGAGGCCTCAACG | CAGGCGTAACAGTCGTAG | |
AAEL006726 | F | GCATTCCTGTTCTCGTTCCC | TGGCTTTGGTTCATTGTG |
R | TGAAGTCACATTTGGCCAGC | TATCCGATGTTGGCTTCC | |
AAEL006841 | F | CATCGGGTGTTGCTTCTACG | ACGGTGCCTATCTGAGAAG |
R | TCAAAGTACACGTGCTGCAG | GGATGCTGATGAACGCTAC | |
AAEL006975 | F | GCCATTTCGATGCCAAAACG | CGTCTGGTGTAGGTGCTAAGTG |
R | TCGACTGAAATCCGGGAACA | TGCTTGTTCTGCCGCTTGC | |
AAEL007144 | F | AACACTTCAACACGTGTCGG | TTCGCATACGGAGTGTTAC |
R | TCGTACACTTCAGCGGAGTT | CCTTGTGGATGTAGTCTCG | |
AAEL007188 | F | GCAGCGCCAATATCTGAACA | TTCGCAGTCGGATTACTTCTTC |
R | TTCCCGCTTCTTCAGGTGAT | TGGTTCTTGGTGATATTCGTAGC | |
AAEL007434 | F | CTGTCCTCGCCCAATGAATG | TGCGTTCTGTTCATAATGGTTAC |
R | CTGCAGTAAATCTCCGCACC | GTCGGGTTTGGTTTCACTCC | |
AAEL007544 | F | ATCGTCTATGGCCGGCTTTA | AGTTCTCCTTCCGACATC |
R | TAGCGCTATGATGTCGTCGT | GTAAGCCGCACATTCATC | |
AAEL007684 | F | AGCGATGCAGGACGAGATTA | CGCCACCAGATGTCCTAATG |
R | CGTGGGCCAGTTTCTTATCG | CAGTTGTTCCGATTGCTTCC | |
AAEL008428 | F | TTGGGCATGCTTCACTGATG | TGTTGGATGATGTTGTGAGATGTG |
R | ATCGTCGGAGTATCGCTGTT | ATCGTCGGAGTATCGCTGTTC | |
AAEL008678 | F | GCCGTTTCCAGGACAACTTT | CCAGTCAGAGGGCGAATG |
R | GTAGTAATCCCGCTCTGCCT | CTTCTCCGTCAGGTCATCC | |
AAEL009047 | F | GGAACGGTGAAATCGATGGG | CACAGAGGAGGAAGTAATTG |
R | TTCACTGCTGTCGTTGTGTG | CACTATTGGACTGCTAACG | |
AAEL009321 | F | CAGCGACGAACCACAATGTA | GTATATGTCGCCTCGGTTC |
R | GCTTATCGCCGATGGTTACC | GGGTTGTATCGGTGTTCC | |
AAEL009357 | F | TCAAGCAAGTGCTGGACAAC | Not completed |
R | TGCCTTCAGGTCGTTCTCTT | ||
AAEL009553 | F | CAGTAGCTTTCCGTCCATGC | GTTGCTCTTCGTCATTATTCC |
R | GACCAGCGGATAAATCGCAG | AAATCCATTGCCATCTTTGC | |
AAEL010639 | F | GCGCACGTTATGATGGGAAT | GCGGAGATGTTACCTTGAAG |
R | TTCCCTTGCATCACATCCCT | GAAACTACCTGGACCTCTGG | |
AAEL011098 | F | TTGGCGAAATTCTGCAAGCT | ACTCATACACTCGTTTCAAG |
R | AGCCGAAACGTTTGCTTCAT | TCCATATCCGAAGCACTC | |
AAEL011310 | F | GGATATGAGACCCGAACCGT | GAAGATGCCGAGCGAGAG |
R | GTTTTCTTCGGTGCCTTCGT | GACCGACCTGGATGGATTC | |
AAEL012096 | F | AATACCAGCACGCTCTCTCA | Not completed |
R | ATTGCATCGGTGGCAAGTTT | ||
AAEL012446 | F | GCTACTTGGATTTGGGCGAC | CGGAGCCAAGGAGGTCATC |
R | ATCGCTTCGGACAGGATGAT | ACAGCAGCAGAAGCAGAGG | |
AAEL013621 | F | TTTGAACCCGGAAAAGGCAG | CCAGAGCAACCGAGAGTATG |
R | TTCGACGAAATCCTCCCACA | CGACGAAATCCTCCCACAG | |
AAEL013723 | F | GATGAAACTGCCGCCACTAG | TTAAGATTGTCACCTTCACC |
R | TTGCCGAACCGTTGGAAAAT | CGTTGTAGATGTTCTGTCC | |
AAEL013737 | F | AAGACTTGGGAAGAGGACGG | TGTAGTTCGGTATCGTTCGG |
R | TTCTCTAGCAGCTGGATCCG | TCGGCATTCCTTCGTTCG | |
AAEL014067 | F | TTGCTCATACGCTCCATTGC | ACAACAGAGCCTAAGACTATC |
R | TTGCTCCTGAACGGTGAGAT | CGACAATCATATTCTCACAGC | |
AAEL014408 | F | GACCGATCCTGCAAAAGTCC | TGGACGATAATGCTCAAC |
R | TTTGCTCCTGGGTGTAGAGG | GAGGCGAATGGAGTTATG |