Figure 7
Expression of miR-281 in female mosquitoes and midguts at 48 hrs after injection of miR-281 antagomiRs. (A) RT-qPCR analysis of the relative expression of miR-281 in female mosquitoes, showing a significant decrease in antagomiR-281-injected mosquitoes compared to that of mock-injected (normal female mosquitoes without injection) and control antagomiR injected mosquitoes. (B) The relative expression of miR-281 in mosquitoes was significantly decreased in the midguts of antagomiR-281-injected mosquitoes. Midguts were dissected from mosquitoes treated as in (A). The RT-qPCR data were analyzed using the 2-Δct method. Data shown represent five (mosquito) or three (midgut) independent experiments with three triplicates. P values are from ANOVA.