Figure 4

Anti- Wolbachia activity against Brugia macrofilariae in SCID mice. (A) % recoveries of Bm macrofilariae (B) Wolbachia loads per female Bm and (C) numbers of released mf per female Bm in BALB/c SCID mice +12 weeks after intraperitoneal infection with 100 Bm L3 and +6 weeks after commencement of 4 week oral 25 mg/kg bid minocycline (MIN) or vehicle control (VC). Error bars indicate median and interquartile range. Dashed horizontal lines in (B) indicate 1 and 2 log reductions compared with median VC levels. Median % reduction in (B) is indicated in parentheses. Data is from an individual experiment (n = 5/group; individual worms pooled per group). Significant differences between groups (Mann Whitney Test) are indicated *P < 0.05, ***P < 0.001.