Isolation and genotyping of viable Toxoplasma gondii from sheep and goats in Ethiopia destined for human consumption

Gebremedhin, Endrias Zewdu; Abdurahaman, Mukarim; Tessema, Tesfaye Sisay; Tilahun, Getachew; Cox, Eric; Goddeeris, Bruno; Dorny, Pierre; De Craeye, Stephane; Dardé, Marie-Laure; Ajzenberg, Daniel

doi:10.1186/1756-3305-7-425

Table 1 Genotyping results of T. gondii DNA with 15 microsatellite markers from 33 isolates collected in sheep and goats of Central Ethiopia and from 14 reference strains collected in America, Africa, Asia, and Europe

From: Isolation and genotyping of viable Toxoplasma gondii from sheep and goats in Ethiopia destined for human consumption

				Microsatellite markers¹
Isolate	Type	Origin	Host	*TUB2*	*W35*	*TgM-A*	*B18*	*B17*	*M33*	*IV.1*	*XI.1*	*M48*	*M102*	*N60*	*N82*	AA	*N61*	*N83*
TgSpEt2	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	NA	176	NA	NA	NA	NA	NA
TgSpEt3	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	211	176	140	123	259	091	308
TgSpEt4	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	211	176	140	123	259	091	308
TgGtEt1	II	W.Shewa	Goat	289	242	207	158	336	169	274	356	225	172	142	117	259	093	308
TgGtEt2	II	W.Shewa	Goat	289	242	207	158	336	169	274	356	235	176	138	115	279	099	312
TgGtEt3	II	W.Shewa	Goat	289	242	207	158	336	169	274	356	235	176	138	115	279	099	312
TgGtEt5	III	E.Shewa	Goat	289	242	205	160	336	165	278	356	213	190	147	113	267	089	312
TgGtEt4	II	W.Shewa	Goat	289	242	207	158	336	169	274	356	237	176	140	113	273	093	312
TgSpEt6	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	213	176	140	115	265	117	308
TgGtEt8	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	211	178	142	109	285	085	310
TgGtEt21²	II	E.Shewa	Goat	NA	242	NA	158	336	NA	NA	356	NA	NA	NA	NA	NA	NA	312
TgGtEt22²	II	E.Shewa	Goat	NA	242	NA	158	NA	NA	274	NA	NA	NA	NA	NA	279	NA	312
TgGtEt12	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	213	176	140	115	261	089	308
TgSpEt10	II	E.Shewa	Sheep	289	NA	207	158	336	169	274	NA	NA	NA	NA	NA	NA	NA	312
TgSpEt28²	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	213	176	140	115	261	089	308
TgGtEt14	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	233	174	140	113	269	097	312
TgGtEt13	II	E.Shewa	Goat	289	242	207	158	336	169	274	NA	NA	NA	140	113	NA	NA	NA
TgSpEt19	Atypical	E.Shewa	Sheep	289	240	201	156	340	163	272	360	209	218	147	127	283	099	342
TgGtEt17	III	E.Shewa	Goat	289	242	205	160	336	165	278	356	213	190	147	113	267	089	312
TgSpEt18	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	211	176	140	109	267	091	308
TgGtEt16	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	211	176	140	109	267	091	308
TgSpEt22	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	219	176	140	111	267	097	310
TgSpEt20	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	213	178	140	115	261	091	308
TgSpEt21	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	211	176	140	109	267	091	308
TgGtEt19	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	215	176	138	117	267	099	312
TgSpEt23	III	E.Shewa	Sheep	289	242	205	160	336	165	278	356	213	190	147	111	271	089	312
TgSpEt25	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	211	176	140	121	267	091	308
TgSpEt26	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	239	176	138	115	281	097	310
TgSpEt27	II	E.Shewa	Sheep	289	242	207	158	336	169	274	356	213	176	140	123	259	091	308
TgGtEt20	II	E.Shewa	Goat	289	242	207	158	336	169	274	356	213	176	140	115	261	089	308
TgSpEt7	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	225	172	142	117	261	093	308
TgSpEt8	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	213	178	140	115	265	089	308
TgSpEt9	II	W.Shewa	Sheep	289	242	207	158	336	169	274	356	NA	NA	140	115	NA	NA	316
GAB5-GAL-DOM01	Africa 1	Gabon	Chicken	291	248	205	160	342	165	274	354	231	166	149	111	277	087	306
CCH002-NIA	Africa 2	Senegal	Human	289	248	205	160	336	165	274	354	225	166	145	111	273	089	308
GAB2-GAL-DOM02	Africa 3	Gabon	Human	291	242	207	160	342	165	278	354	223	166	142	111	277	097	310
ENT	I	France	Human	291	248	209	160	342	169	274	358	209	166	145	119	267	087	306
ME49	II	USA	Sheep	289	242	207	158	336	169	274	356	215	174	142	111	265	091	310
NED	III	France	Human	289	242	205	160	336	165	278	356	209	190	147	111	267	091	312
VAND	Atypical	French Guiana	Human	291	242	203	162	344	167	276	356	217	170	142	113	277	091	308
GUY-CAN-FAM01	Caribbean 1	French Guiana	Dog	291	242	205	162	342	165	278	356	213	164	142	109	265	087	312
TgCtPRC04	Chinese 1	China	Cat	293	242	211	160	336	169	274	354	215	172	145	123	281	093	308
TgCatBr01	BR II	Brazil	Cat	289	242	205	160	342	165	278	358	233	164	147	111	316	089	308
TgCatBr03	BR III	Brazil	Cat	289	242	205	160	348	165	278	356	213	190	142	111	263	113	312
TgCatBr05	Atypical	Brazil	Cat	291	242	205	160	362	165	278	356	237	174	140	111	265	089	314
TgCatBr15	Atypical	Brazil	Cat	289	242	205	162	344	165	278	358	225	164	142	111	263	105	312
TgCkBr81	BR IV	Brazil	Chicken	291	246	207	162	362	169	272	358	217	164	142	105	322	081	314

W. Shewa = West Shewa Zone, E. Shewa = East Shewa Zone.
¹NA, not amplified, ² cyst negative but nPCR positive.

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ISSN: 1756-3305

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