Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?

Table 1 Species-specific ITS rDNA primers

Species [GenBank Acc.Number]	Annealing temperature	Primer name	Primer sequence 5’-3’	PCR product size (nt)	Amplified region
C. labracis [JQ694148]	66.6°C	Caino_F	ACGTGCAGCTCATGACACGG	301	ITS1
		Caino_R	TCAGTCAAGCCAGGGGAAGG
M. obovata [JQ694145]		Macv_F	CCCGAGGCACTCAAAGACTG	537	ITS1
		Macv_R	TCAGTCGAGCCCAGGATAGG
M. novaezealandensis [KJ540203]	64°C	Maritr_F	TTGACATTCGGCCGGGGTGC	214	ITS2
M. novaezealandensis [KJ540203]		Maritr_R	ACCGGCCTAAAGCGCACAGA
Philophthalmus sp. [KJ540204]		Philsp_F	CGTGAGAGATCACGCGAGG	352	ITS2
		Philsp_R	TGTGCGCCTCACCAAGTGAG

Species-specific ITS rDNA primers designed in this study for amplification of i) C. labracis and M. obovata, infecting G. adansonii snails from the Ebro Delta (Spain) and ii) M. novaezealandensis and Philophthalmus sp., infecting Z. subcarinatus snails from Otago Habour (New Zealand).

ISSN: 1756-3305