Immunization regimena | CD4+(%)b | CD8+(%)b | CD4+/CD8+ |
---|
PBS | 26.71±0.22 | 18.67±0.92 | 1.43±0.23 |
pcDNA3.1 | 27.62±0.25 | 24.71±0.85 | 1.12±0.29 |
pSAG1 | 26.45±0.34 | 26.37±0.98 | 1.00±0.35 |
pSAG1/SAG3 | 24.68±0.14 | 35.01±0.88 | 0.70±0.16 |
pSAG1/SAG3-CTXA2/B | 25.15±0.23 | 38.55±0.94 | 0.65±0.24 |
- a Mice were immunized by i.m. route on day 0 and day 14 and day 28 with PBS, pcDNA3.1, pSAG1, pSAG1/SAG3, pSAG1/SAG3-CTXA2/B.
- b The splenocytes culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITC-labeled anti-mouse CD8+ monoclonal antibody and PE-labeled anti-mouse CD4+ monoclonal antibody, T lymphocyte subsets were measured using flow cytometry.