Figure 2

Relative expression analysis after miRNA interference with three miRNAs in developmental stages of Ae. albopictus by Bulge-loop miRNA qPCR. (A) Relative gene expression of miR-1891 in adults analyzed by ANOVA (F = 180.22, p < 0.001) (B) Relative expression of miR-286b in embryo analyzed by ANOVA (F = 32.908, p < 0.001) (C) Relative expression of miR-2942 in larvae analyzed by ANOVA (F = 19.648, p = 0.002). * A significant decrease (p < 0.001) of expression in inhibitor group compared with the negative control group (analyzed by statistical method of ANOVA by LSD). ** No significant difference (p > 0.05) in expression level between inhibitor and negative control group (analyzed by statistical method of ANOVA, LSD). In all miRNA qPCR quantifications, the relative gene expressions from normal controls are calibrated to 1 (not shown in graph). Error bars show the SEM of three biological replicates, each containing one adult male/female.