Table 1 Sequences of the primers used to amplify the MSP-1 and MSP-2 genes of P. falciparum isolates from Pahang, Malaysia

Primary PCR

MSP-1

M1-OF

5^- -CTAGAAGCTTTAGAAGATGCAGTATTG-3^-

M1-OR

5^- -CTTAAATAGTATTCTAATTCAAGTGGATCA-3^-

MSP-2

M2-OF

5^- -ATGAAGGTAATTAAAACATTGTCTATTATA-3^-

M2-OR

5^- -CTTTGTTACCATCGGTACATTCTT-3^-

Secondary PCR

MSP-1

M1-KF

5^- -AAATGAAGAAGAAATTACTACAAAAGGTGC-3^-

M1-KR

5^- -GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3^-

M1-MF

5^- -AAATGAAGGAACAAGTGGAACAGCTGTTAC-3^-

M1-MR

5^- -ATCTGAAGGATTTGTACGTCTTGAATTACC-3^-

M1-RF

5^- -TAAAGGATGGAGCAAATACTCAAGTTGTTG-3^-

M1-RR

5^- -CATCTGAAGGATTTGCAGCACCTGGAGATC-3^-

MSP-2

M2-FCF

5^- -AATACTAAGAGTGTAGGTGCARATGCTCCA-3^-

M2-FCR

5^- -TTTTATTTGGTGCATTGCCAGAACTTGAAC-3^-

M2-ICF

5^- -AGAAGTATGGCAGAAAGTAAkCCTYCTACT-3^-

M2-ICR

5^- -GATTGTAATTCGGGGGATTCAGTTTGTTCG-3^-