Figure 2

Time (min:sec) to perform duplicate Kato-Katz thick smears or the FLOTAC dual technique in an epidemiological survey on soil-transmitted helminth infections. The area of each segment of the circles is proportional to the amount of time used in each working step. S1: Driving to school. S2: Distribution of pre-labeled stool container, and collection of stool samples. S3: Driving to laboratory. K1: Labeling microscope slides and preparing duplicate Kato-Katz thick smears. K2: Quantitative microscopic reading for hookworm eggs K3: Quantitative microscopic reading for A. lumbricoides and T. trichiura eggs. K4/F9: Data entry (name, age, sex and school grade). K5/F10: Data entry (egg counts from Kato-Katz/FLOTAC). K6/F11: Washing and preparing materials for the next day. F1: Weighting ~1 g of stool and homogenization in 10 ml 5% formaldehyde. F2: Filtering homogenized stool and filling into two Falcon tubes. F3: Centrifugation of the Falcon tubes, discarding supernatant and filling flotation solutions into tubes. F4: Assembling of the FLOTAC apparatus. F5: Filling FLOTAC apparatus with homogenized suspension (pellet and flotation solution). F6: Centrifugation and afterwards translation of FLOTAC apparatus. F7: Reading chamber 1 quantitatively for soil-transmitted helminth eggs. F8: Reading chamber 2 quantitatively for soil-transmitted helminth eggs.