Table 1 Primers used in this study
From: Epidemiology and genetic diversity of Anaplasma ovis in goats in Corsica, France
Target gene | Primer/probe (5'-3') | Type of PCR | Tm (°C)a | Fragment length (bp) |
|---|---|---|---|---|
msp4 | TCATTCGACATGCGTGAGTCA | Real-time | 60 | 92 |
TTTGCTGGCGCACTCACATC | 60 | |||
FAM-AGCAGAGAGACCTCGTATGTTAGAGGC-BQ1 | 70 | |||
gltA | GCCGACTTTGTTGCCACTGT | Qualitative | 58.7 | 760 |
TCCAACCGCCCTTAGCACAA | 59.4 | |||
msp4 | CCCAGCGTTTCCCTCTGTTA | 57.1 | 497 | |
GAGTCCGTGGTAGAACCCAC | 57.3 |