Table 3 Gene expression analysis performed in mosquito lysates by direct qPCR and matched purified eluates after nucleic acid extraction (n = 4 susceptible vs n = 4 resistant). P values were calculated by the pair wise fixed reallocation randomization test

CYP6P3 (A)

4.68 (2.67–8.81)

0.001

4.10 (3.35–5.23)

0.020

CYP6M2 (A)

4.57 (3.18–7.19)

0.002

2.70 (2.18–3.40)

0.022

CYP9K1 (B)

4.66 (3.40–7.70)

0.002

4.45 (4.12–4.80)

<0.001

CYP6P4 (B)

2.29 (1.31–3.71)

0.018

1.67 (1.33–2.11)

<0.001

CYP6Z1 (C)

2.98 (2.47–3.84)

0.009

1.85 (1.44–2.22)

<0.001

GSTE2 (C)

13.6 (9.64–18.4)

0.008

9.36 (7.96–11.9)

<0.001

CYP6P1 (D)

1.32 (0.968–1.90)

0.060

1.64 (0.752–3.57)

0.215

CYP4G16 (D)

2.81 (1.61–4.26)

0.008

1.91 (1.45–2.46)

<0.001