Table 2 Multilevel validation of multiplex detox assays by comparing corresponding expression analysis results to published data and the “gold-standard” singleplex RT-qPCR method. n =3 replicates were used for the multiplex detox assays and singleplex RT-qPCR analysis and P-values were calculated by the pair wise fixed reallocation randomization test. For the correlation analysis P-values were calculated by Pearson’s correlation coefficient (r) test

CYP6P3

VK7 vs NG

3.30 (P = 0.013) [42]

3.53 (1.44–8.14) P < 0.001

6.58 (1.68–26.39) P < 0.001

-13.60 (-127–100)

0.703 (P = 0.001)

TIA vs NG

29.5 (P = 0.005) [27]

20.7 (9.30–34.2) P = 0.033

15.1 (6.83–40.9) P < 0.001

AKR vs KIS

– [23, 35]

13.6 (5.77–29.4) P = 0.024

15.3 (6.59–32.4) P < 0.001

CYP6M2

VK7 vs NG

1.80 (P = 0.0044) [42]

6.58 (3.92–8.94) P = 0.031

3.73 (1.98–9.48) P < 0.001

2.32 (-116–120)

0.941 (P < 0.001)

TIA vs NG

6.41 (P = 0.015) [27]

4.46 (0.942–21.1) P = 0.059

4.99 (0.72–34.6) P = 0.103

AKR vs KIS

– [23, 35]

21.5 (5.36–59.36) P = 0.006

31.3 (13.1–99.0) P < 0.001

CYP9K1

VK7 vs NG

1.40 (P = 0.059) [42]

1.28 (0.958–1.71) P = 0.094

2.94 (0.942–9.20) P = 0.063

-56.47 (-151–38.4)

0.809 (P = 0.003)

TIA vs NG

4.33 (P = 0.007) [27]

2.01 (1.72–2.32) P < 0.001

4.60 (1.80–18.3) P < 0.001

AKR vs KIS

– [23, 35]

8.20 (6.78–10.9) P = 0.006

9.28 (2.48–20.9) P = 0.026

CYP6P4

VK7 vs NG

2.11 (P = 0.11) [42]

6.77 (5.20–8.49) P = 0.014

8.97 (7.50–10.7) P < 0.001

39.65 (-123–202)

0.856 (P < 0.001)

TIA vs NG

14.4 (P = 0.0047) [27]

13.0 (7.92–18.4) P < 0.001

8.33 (4.05–17.6) P = 0.033

AKR vs KIS

– [23, 35]

8.54 (5.25–12.1) P < 0.001

2.73 (1.23–6.73) P = 0.043

CYP6Z1

RSP vs KIS

3.50 (P < 0.05) [43]; 1.31 (P < 0.05) [44]

3.03 (2.58–3.38) P = 0.036

2.69 (1.80–4.79) P < 0.001

-35.86 (-101–29.3)

0.512 (P = 0.03)

ZANU vs KIS

> 2.0 (P < 0.05) [44]

3.27 (1.20–5.23) P = 0.045

3.25 (0.81–12.98) P = 0.095

TIA vs NG

1.21 (P > 0.05) [27]

2.14 (0.625–4.68) P = 0.173

2.52 (0.819–7.76) P = 0.11

VK7 vs NG

1.40 (P = 0.013) [42]

0.874 (0.747–1.02) P = 0.09

3.31 (1.02–10.75) P = 0.050

AKR vs KIS

– [23, 35]

6.53 (2.52–9.97) P = 0.041

12.0 (3.55–77.4) P = 0.001

GSTE2

ZANU vs KIS

7.80 (P < 0.05) [45] 3.90 (P < 0.05) [44]

57.8 (52.3–67.5) P = 0.041

22.7 (4.24–328.6) P < 0.001

20.58 (-50.5–91.7)

0.678 (P = 0.003)

RSP vs KIS

2.36 (P < 0.05) [44]

30.7 (26.6–34.3) P = 0.017

16.5 (1.30–161.3) P < 0.001

TIA vs NG

0.649 (P > 0.05) [27]

0.652 (0.406–1.05) P = 0.08

0.735 (0.340–1.58) P = 0.382

VK7 vs NG

0.909 (P = 0.75) [42]

1.30 (0.891–1.90) P = 0.174

1.015 (0.403–2.78) P = 0.899

AKR vs KIS

– [23, 35]

27.5 (6.90–34.5) P = 0.034

49.5 (12.5–347.3) P < 0.001

CYP6P1

VK7 vs NG

2.20 (P = 0.048) [42]; 1.70 (P < 0.05) [46]

1.55 (1.21–1.84) P = 0.024

2.64 (1.75–3.33) P < 0.001

-59.84 (-111– -8.5)

0.703 (P = 0.002)

TIA vs NG

1.84 (P = 0.0037) [27]

2.85 (2.56–3.28) P = 0.023

4.48 (2.54–6.91) P < 0.001

AKR vs KIS

– [23, 35]

4.24 (0.886–9.79) P = 0.066

10.3 (2.17–44.9) P = 0.036

CYP4G16

VK7 vs NG

1.90 (P < 0.05) [46]; 1.10 (P = 0.59) [42]

2.03 (1.67–2.51) P = 0.019

2.63 (1.75–3.33) P < 0.001

-51.21 (-109–7.1)

0.708 (P < 0.001)

TIA vs NG

0.455 (P > 0.05) [27]

1.20 (0.760–1.75) P = 0.319

2.55 (0.980–10.9) P = 0.052

AKR vs KIS

– [23, 35]

2.18 (1.29–3.46) P = 0.023

3.87 (1.03–23.2) P = 0.050