Table 1 Primers used for microfluidic real-time PCR
Species | Target gene | Forward primer (5'-3') | Reverse primer (5'-3') | Reference |
|---|---|---|---|---|
Babesia spp., Theileria spp., Hepatozoon spp. | 18S rRNA | GTGAAACTGCGAATGGCTCATTAC; GGCTCATTACAACAGTTATAGTTTATTTG | AAGTGATAAGGTTCACAAAACTTCCC; CGGTCCGAATAATTCACCGGAT | [66] |
Borrelia spp. | flaB | GCAGTTCARTCAGGTAACGG; GCATCAACTGTRGTTGTAACATTAACAGG | GCAATCATAGCCATTGCAGATTGT; ACATATTCAGATGCAGACAGAGGT | [67] |
Anaplasma spp., Ehrlichia spp. | 16S rRNA | GAACGAACGCTGGCGGCAAGC; TGCATAGGAATCTACCTAGTAG | AGTAYCGRACCAGATAGCCGC; AGTAYCGRACCAGATAGCCGC | [68] |
Coxiella-like endosymbiont | 16S rRNA | CGTAGGAATCTACCTTRTAGWGG; TGAGAACTAGCTGTTGGRRAGT | ACTYYCCAACAGCTAGTTCTCA; GCCTACCCGCTTCTGGTACAATT | [69] |
Rickettsia spp. | Citrate synthase | GGGGGCCTGCTCACGGCGG | ATTGCAAAAAGTACAGTGAACA | [70] |