Fig. 5

Inflammatory reaction grades and skin lesions at two (a) and four weeks (b) post-challenge. The colored lines indicate the group average. Key: PBS, immunized with 1 ml 0.01 M PBS each time; Quil A, immunized with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) each time; vector protein, immunized with 100 μg (first time) and 200 μg (second time) purified pET32a(+) vector protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS); rSsCLP5, rSsCLP5’, and rSsCLP5” refer to the test groups immunized with 100 μg then 200 μg purified rSsCLP5 protein with 1 ml QuilA saponin at concentration of 1 mg/ml (dissolved in PBS) at the first and second immunizations. The grades of inflammatory reaction and skin lesions in the rSsCLP5 immunized groups (rSsCLP5, rSsCLP5’, and rSsCLP5”) were significantly lower compared to the control groups at week 2 post-challenge (F_(5,63) = 13.575, P < 0.0001) and at week 4 post-challenge (F_(5,63) = 23.38, P < 0.0001)