Fig. 1

SDS-PAGE and Western blot analyses of the purified rFgRab10 protein from the sonicated culture sediment of E. coli. a rFgRab10 protein was resolved on 12% acrylamide gel and stained with Coomassie blue. Lane M: prestained protein ladder in kilodaltons; Lane P: purified recombinant protein, rFgRab10, which appeared as a single band of ~39 kDa. b rFgRab10 protein was run under non-reducing conditions, and the immunoreaction was visualized using chemiluminescent HRP substrate and X-ray film. Lane M: prestained protein ladder; Lane P: loaded with rFgRab10 protein expressed from E. coli. Serum from Fasciola-infected sheep detected a single band of ~39 kDa; Lane C1: loaded with protein expressed from sonicated culture supernatant of E. coli transformed with empty pET-SUMO vector without rFgRab10 gene insert, which did not react with goat serum containing anti-F. gigantica IgG antibodies; Lane C2: loaded with rFgRab-10 protein expressed from E. coli that did not react with the serum of healthy uninfected goats