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Table 2 Treatment conditions to induce B. bigemina sexual stages. All cultures were in the presence of 5% CO2. Bold indicates sexual stage induction

From: Identification of a putative methyltransferase gene of Babesia bigemina as a novel molecular biomarker uniquely expressed in parasite tick stages

Condition

Inducer

Concentrations (mM)

26 °C

TCEP

0

10

20

25

30

40

TCEP+ XA

0

10

20

25

30

40

37 °C

TCEP

0

10

20

25

30

40

TCEP+ XA

0

10

20

25

30

40

26 °C

DTT

0

10

20

25

30

40

DTT+ XA

0

10

20

25

30

40

37 °C

DTT

0

10

20

25

30

40

DTT+ XA

0

10

20

25

30

40

  1. Abbreviations: TCEP, Tris 2-carboxyethyl phosphine; DTT, dithiothreitol; XA, xanthurenic acid