Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis

Table 2 Enzyme kinetic constants of recombinant GSTs in the presence of different acaricides

Acaricide	Class	Recombinant HlGST			Recombinant HlGST2
Acaricide	Class	V _max	K _m	Inhibition	V _max	K _m	Inhibition
None		11.70 ± 1.92	0.82 ± 0.14		14.72 ± 0.56	0.61 ± 0.20
Flumethrin	Synthetic pyrethroids	4.26 ± 0.30*	0.48 ± 0.07*	UC	5.85 ± 0.73	1.70 ± 0.92	None
Cypermethrin	Synthetic pyrethroids	3.61 ± 1.65*	0.38 ± 0.04*	UC	2.20 ± 0.64*	0.36 ± 0.12	NC
Chlorpyrifos	Organophosphates	9.52 ± 4.21	0.55 ± 0.24	None	8.00 ± 1.08*	0.35 ± 0.25	NC
Ethion	Organophosphates	8.36 ± 0.87	0.58 ± 0.15	None	15.55 ± 1.02	0.37 ± 0.07	None
Coumaphos	Organophosphates	6.35 ± 2.40	0.48 ± 0.10*	None	9.68 ± 3.38	1.17 ± 0.31	None
Diazinon	Organophosphates	18.38 ± 5.43	1.51 ± 0.78	None	26.13 ± 5.33	1.59 ± 0.58	None
Amitraz	Formamidine	15.04 ± 1.66	0.87 ± 0.25	None	10.10 ± 2.55	0.51 ± 0.09	None
Ivermectin	Avermectin	10.94 ± 5.10	0.74 ± 0.36	None	14.64 ± 3.47	0.74 ± 0.31	None

ISSN: 1756-3305