Fig 5From: Mutual interactions of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella with cultured poultry macrophagesMacrophage mRNA relative cytokine expression by infection group. Normalization of data was performed on housekeeping genes GAPDH and G6PDH. The mRNA transcriptional levels of TNF-α, IL-6, IL-10, IL-12, IFN-γ, iNOS were determined by RT-qPCR. Displayed x-fold change values were calculated based on negative control (NC) group replicates (n = 6). Statistical comparison was performed between the CI group and other groups (*P < 0.05). Abbreviations: TH, high-dose infection with T. gondii; TL, low-dose infection with T. gondii; EH, high-dose infection with E. tenella; EL, low-dose infection with E. tenella; CI, co-infected groupBack to article page