Fig. 2

Overview of the relationships between European B. afzelii STs detected in human samples using goeBURST. CCs were identified at the DLV threshold. The 149 STs clustered into eight major complexes (consisting of three or more STs), five minor complexes (consisting of two STs), and 11 singletons with no connection with any other STs. The circled fraction of CC0 corresponds to CC1’ when CCs were defined at the SLV threshold. Colored lines between STs indicate in descending order of certainty: black lines inferred without tiebreak rules, blue lines inferred using tiebreak rule 1 (number of SLVs), green lines using tiebreak rule 2 (number of DLV), and yellow lines using tiebreak rules 4 or 5 (frequency found on the data set and ST number, respectively). STs connected by gray lines are DLVs. The inferred founders of CCs are outlined in light green and subgroup founders in dark green. Circle size corresponds to MLST sample size. Circle color fractions refer to clinical manifestations associated with STs (see the legend)