Extracellular release of virulence factor major surface protease via exosomes in Leishmania infantum promastigotes

Table 1 MSP proteins identified by LC-MS/MS from exosomes released by L. infantum promastigotes

	Protein name	Class	Predicted size (kDa)	Accession number	Leishmania spp. with annotation	Mean coverage (%)	No. of peptides involved in class identification
1	LinJ.10.520	S	63	CAM66068	L. infantum	37	3
2	LinJ.10.530	S	63.5	CAM66064	L. infantum	39	5
3	GP63_LEIDO4	S	64	AAA29237	L. donovani	48	5
4	LcMSPL1	L	63.8	P15706	L. infantum	40	7
5	GP63_LEIDO	L	63	AAA29244	L. donovani	29	4
6	GP63_LEIAM	L	63	Q27673	L. amazonensis	8	2
7	GP63_LEIMA	L	64	P08148	L. major	12	3
8	LinJ.28.600^a	C	60.6	CBZ08848	L. infantum	21	5
9	LinJ.10.510	C	69	CAM66067	L. infantum	30	3
10	LmjF.10.470	C	69	Q4QHH0	L. major	16	2
11	LmxM.10.460	C	70	XP_003872882	L. mexicana	10	2
12	GP63_LEIME	C	69	P43150	L. mexicana	5	1
13	GP63_LEITR	C	70	Q8MNZ1	L. tropica	6	1
14	GP63_LEIDO2	C	60	CBY93846	L. donovani	9	0
15	GP63_LEIDO3	C	61	CBY93851	L. donovani	21	2
16	GP63_LEIDO5	C	39	CAC37955	L. donovani	15	2
17	GP63_LEIDO6	C	40	CAC37953	L. donovani	28	1

Note: The species column indicates the Leishmania species in which the protein has been annotated in the database. The mean % coverage is the proportion of amino acids identified per allocated protein and gives an indication of the reliability of the results. Results were restricted to a protein false discovery rate (FDR) of < 0.1% with peptide and protein confidence of 95% and at least two unique peptides required
^aThis was previously renamed as MSP-like protein (MLP) [29]

ISSN: 1756-3305