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Table 3 Summary of the characteristics of real-time PCR assays targeting Leishmania sequences other than kDNA or rDNA, in chronological order

From: Real-time PCR applications for diagnosis of leishmaniasis

Target sequence

Geographical region

Type of sample

Assay type

Assay chemistry

Typing

Reference

DNA pol

Old World

Liver biopsy

Quantitative

Fluorescent probe

No

[6]

GPI

Old World/ New World

Skin biopsy

Qualitative

Fluorescent probe

Yes

[36]

DNA pol

New World

Sand flies

Quantitative

Fluorescent probe

No

[85]

GP63

Old World

Mice tissues

Quantitative

Intercalating dye

Yes

[68]

G6PD

New World

Biopsy

Quantitative

Intercalating dye/ Fluorescent probe

Yes

[35]

DNA pol

New World

Sand flies

Quantitative

Intercalating dye

No

[32]

Alpha-tubulin, DNA pol, Spliced leader, MSP-associated gene (MAG), SIDER repeat, GPI, HSP70, splice leader-associated retrotransposons (SLACS)

Old World/ New World

Blood/ skin biopsy

Quantitative

Intercalating dye/ Fluorescent probe

Yes

[20]

tryparedoxin peroxidase

Old World

Skin biopsy

Qualitative

Intercalating dye

Yes

[37]

DNA pol

New World

Skin biopsy/ bone marrow/ blood/ conjunctival swab

Quantitative

Fluorescent probe

No

[34]

DNA pol

New World

Bone marrow/ skin biopsy/ nasal, oral, ear swab

Quantitative

Fluorescent probe

No

[33]

MPI, 6PGD

New World

Scrapings/ skin biopsy

Qualitative

Fluorescent probe

Yes

[9]

G6PD

New World

Biopsy

Quantitative

Intercalating dye

No

[13]

HSP70

New World

Parasite cultures

Qualitative

Intercalating dye

Yes

[27]

AAP3

Old World/ New World

Mouse tissues

Quantitative

Fluorescent probe

No

[116]

REPL repeats (L42486.1)

Old World

Blood

Quantitative

Fluorescent probe

Specific for L. infantum and L. donovani

[117]

LinJ31

Old World/ New World

Lymph node/ bone marrow aspirate/ tissue samples

Quantitative

Fluorescent probe

Specific for subgenus Leishmania

[118]

CPB

Old World

Cutaneous biopsy

Qualitative

Fluorescent probe

Yes

[8]

HSP70

Old World/ New World

Biopsy/ sand flies

Qualitative

Intercalating dye

Yes

[29]

IPPP, RNase III

New World

Sand flies

Quantitative

Intercalating dye

Yes

[119]

REPL repeats (L42486.1)

Old World

Blood/ skin

Quantitative

Fluorescent probe

Specific for L. infantum and L. donovani

[69]

HSP70

New World

Parasite cultures

Quantitative

Intercalating dye

No

[30]