From: Real-time PCR applications for diagnosis of leishmaniasis
Target sequence | Geographical region | Type of sample | Assay type | Assay chemistry | Typing | Reference |
---|---|---|---|---|---|---|
DNA pol | Old World | Liver biopsy | Quantitative | Fluorescent probe | No | [6] |
GPI | Old World/ New World | Skin biopsy | Qualitative | Fluorescent probe | Yes | [36] |
DNA pol | New World | Sand flies | Quantitative | Fluorescent probe | No | [85] |
GP63 | Old World | Mice tissues | Quantitative | Intercalating dye | Yes | [68] |
G6PD | New World | Biopsy | Quantitative | Intercalating dye/ Fluorescent probe | Yes | [35] |
DNA pol | New World | Sand flies | Quantitative | Intercalating dye | No | [32] |
Alpha-tubulin, DNA pol, Spliced leader, MSP-associated gene (MAG), SIDER repeat, GPI, HSP70, splice leader-associated retrotransposons (SLACS) | Old World/ New World | Blood/ skin biopsy | Quantitative | Intercalating dye/ Fluorescent probe | Yes | [20] |
tryparedoxin peroxidase | Old World | Skin biopsy | Qualitative | Intercalating dye | Yes | [37] |
DNA pol | New World | Skin biopsy/ bone marrow/ blood/ conjunctival swab | Quantitative | Fluorescent probe | No | [34] |
DNA pol | New World | Bone marrow/ skin biopsy/ nasal, oral, ear swab | Quantitative | Fluorescent probe | No | [33] |
MPI, 6PGD | New World | Scrapings/ skin biopsy | Qualitative | Fluorescent probe | Yes | [9] |
G6PD | New World | Biopsy | Quantitative | Intercalating dye | No | [13] |
HSP70 | New World | Parasite cultures | Qualitative | Intercalating dye | Yes | [27] |
AAP3 | Old World/ New World | Mouse tissues | Quantitative | Fluorescent probe | No | [116] |
REPL repeats (L42486.1) | Old World | Blood | Quantitative | Fluorescent probe | Specific for L. infantum and L. donovani | [117] |
LinJ31 | Old World/ New World | Lymph node/ bone marrow aspirate/ tissue samples | Quantitative | Fluorescent probe | Specific for subgenus Leishmania | [118] |
CPB | Old World | Cutaneous biopsy | Qualitative | Fluorescent probe | Yes | [8] |
HSP70 | Old World/ New World | Biopsy/ sand flies | Qualitative | Intercalating dye | Yes | [29] |
IPPP, RNase III | New World | Sand flies | Quantitative | Intercalating dye | Yes | [119] |
REPL repeats (L42486.1) | Old World | Blood/ skin | Quantitative | Fluorescent probe | Specific for L. infantum and L. donovani | [69] |
HSP70 | New World | Parasite cultures | Quantitative | Intercalating dye | No | [30] |