Fig. 1

Schematic diagram of GFP-expressing plasmid (pBS-EGRADE) construct and fluorescence microscopy images of stably expressing GFP B. gibsoni. a Plasmid construct of pBS-EGRADE showing the recombination sites for integration into ef-1α locus by double cross-over homologous recombination. The restriction site for linearization (Kpn I) is shown. b Fluorescence microscopy images of stable GFP-expressing B. gibsoni. Merged panel shows overlap of GFP and Hoechst (parasite nuclei) fluorescence. The parasite nucleus was stained with Hoechst 33342