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Table 1 Results obtained by using DNA isolated with QIAgen columns with the different instruments in Experiment 1

From: Evaluation of fluorimetry and direct visualization to interpret results of a loop-mediated isothermal amplification kit to detect Leishmania DNA

Instrument

L. infantum 10-fold DNA dilutions of parasite equivalents/μl

 

103

102

10

1

10-1

10-2

10-3

10-4

10-5

10-6

10-7

LF-160

Pos

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Neg

Neg

Neg

Genie III®

Pos

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Neg

Neg

Neg

Tp (min:sec)

13:30

14:30

16:15

17:45

21:45

24:00

34:15

0

0

0

0

ESEQuant TS2.2

Pos

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Neg

Neg

Neg

LnPCR

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Neg

Neg

Neg

Neg

qPCR

Pos

Pos

Pos

Pos

Pos

Pos

Neg

Neg

Neg

Neg

Neg

Ct

19.1

22.5

25.6

28.4

30.7

32.1

Neg

Neg

Neg

Neg

Neg

  1. Abbreviations: Pos positive, Neg negative